LOCUS       sequence-116170-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for S aureus Cas9: 
            CAG-humanSaCas9-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: BPK2139
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..31
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(121..186)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(193..213)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtccccataa tttttggcag agggaaaaag atctcagtgg tatttgtgag ccagggcatt
       61 ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtgc ggccgcttta
      121 cttgtcatcg tcatccttgt aatcgatgtc atgatcttta taatcaccgt catggtcttt
      181 gtagtcgctc gagactttcc tcttcttctt gggggatcca ccttttttaa tgatttgtgg
      241 gtgcttcttt gacttcactt catagagatt ccccaggata tcggtagagt acttttttat
      301 tgactgagtt ttagatgcga ttgtcttgat gatgcgaggc ggccgtttat cattcatgtt
      361 ttcgagatac tcccggtagg taatgtctat catattgact tcgatcctgt tcagcaagtc
      421 attgttcaca ccaatgacgc gataaagctc tccattgatc ttgatgagat cgttattata
      481 gaaggaagcg atgaactcgg cctgattgga gatcttcttc agcttcttcg cctcctcata
      541 gcactttgag tttacttcat aatagttctc ctttttgatc acgtccagat ttttcacggt
      601 gacgaattta taaaccccat tatccaggta cacgtcaaat ctataaggct tcagggagag
      661 cttcacaacc ttgtttcgag aattgg
//
