LOCUS       sequence-116171-         217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for S aureus Cas9: 
            CAG-humanSaCas9-NLS-3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: BPK2139
FEATURES             Location/Qualifiers
     source          1..217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttgacattg ggataaccag cgttggctac ggaattattg attatgagac acgcgatgtg
       61 attgacgccg gggttaggct gttcaaagag gccaacgttg aaaacaacga gggaagacgg
      121 agtaagcgcg gagcaagaag actcaagcgc ngacggagac atcggattcn nagggtgaaa
      181 aagctgctct tcgattacaa tctcctgacc gatcata
//
