LOCUS       sequence-116173-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for S. thermophilus1 Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP1673
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        118..136
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    137..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
       61 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa
      121 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctgtagaa ataattttgt
      181 ttaactttaa taaggagata taccatgggc agcgacctgg tgctgggcct ggacatcggc
      241 atcggcagcg tgggcgtggg catcctgaac aaggtgaccg gcgagatcat ccacaagaac
      301 agtcgcatct tccctgctgc tcaggctgag aacaacctgg tgcgccgcac caaccgccag
      361 ggtcgccggc ttgctcgccg caagaagcac cggcgcgtgc gcctgaaccg cctgttcgag
      421 gagagcggcc tgatcaccga cttcaccaag atcagcatca acctgaaccc ctaccagctg
      481 cgcgtgaagg gcctgaccga cgagctgagc aacgaggagc tgttcatcgc cctgaagaac
      541 atggtgaagc accgcggcat cagctacctg gacgacgcca gcgacgacgg caacagcagc
      601 gtgggcgact acgcccagat cgtgaaggag aacagcaagc agctggagac caagaccccc
      661 ggccagatcc agctggagcg ctaccagacc tacggccagc tgcgcggcga cttcaccgtg
      721 gagaaggacg gcaagaagca ccgcctgatc aacgtgttcc ccaccagcgc ctaccgcagc
      781 gaggccctgc gcatcctgca gacccagcag gagttcaacc cccagatcac cgacgagttc
      841 atcaaccgct acctggagat cctgaccggc aagcgcaagt actaccacgg
//
