LOCUS       sequence-116177-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for SpCas9 VQR variant: 
            CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP469
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact
       61 atagggagag ccgccaccat ggataaaaag tattctattg gtttagacat cggcactaat
      121 tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg
      181 ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat
      241 agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt
      301 cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac
      361 gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa
      421 cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg
      481 atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc
      541 tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta
      601 aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag
      661 ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc
      721 cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa
      781 aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa tttt
//
