LOCUS       sequence-116181-         497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for SpCas9 VRER variant: 
            CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER 
            variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP1101
FEATURES             Location/Qualifiers
     source          1..497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
       61 cactataggg agagccgcca ccatggataa aaagtattct attggtttag acatcggcac
      121 taattccgtt ggatgggctg tcataaccga tgaatacaaa gtaccttcaa agaaatttaa
      181 ggtgttgggg aacacagacc gtcattcgat taaaaagaat cttatcggtg ccctcctatt
      241 cgatagtggc gaaacggcag aggcgactcg cctgaaacga accgctcgga gaaggtatac
      301 acgtcgcaag aaccgaatat gttacttaca agaaattttt agcaatgaga tggccaaagt
      361 tgacgattct ttctttcacc gtttggaaga gtccttcctt gtcnaagagg acaagaaaca
      421 tgancggnac cccatctttg gaaacatagt agatgaggtg gcatatcatg aaaagtaccc
      481 aacgatttat cacctct
//
