LOCUS       sequence-116216-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral 
            vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, 
            Meyer AJ, Dick TP
  TITLE     Real-time imaging of the intracellular glutathione redox potential.
  JOURNAL   Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008
            May 11.
  PUBMED    18469822
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1212"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "553-9"
COMMENT     Sequence Label: pLPCX mito Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..48)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(183..695,696..698))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     376..395
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     635..656
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
       61 tcgttggggt ctttgctcag ggcggagcag gtgctcaggt agtggttgtc gggcagcagc
      121 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
      181 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
      241 atgatataga cgttgtggca gttgtagttg tactccagct tgtgccccag gatgttgccg
      301 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      361 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      421 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      481 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
      541 agcttgccgg tggtggagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      601 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      661 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacga attctcctcc tgatcctcct
      721 cctcctgat
//
