LOCUS       sequence-116227-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 10-12 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX4-40
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(395..412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(395..411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(404..426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      475..561
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      653..680
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcggccatg gtcctggcac agcaccggca acagccgctg caccgtttcg
      121 agcgcttgct tgccgccacc gttgctggcg atagccacca cttggtccgg ggtcaggcca
      181 tggtcctggc acagcaccgg caacagccgc tgcaccgttt cgagcgcttg cttgccgcca
      241 tcgtggctgg cgatagccac cacttggtcc ggagtcaggc catggtcctg gcacagcacc
      301 ggcaacagcc gctgcaccgt ttcgagcgct tgcttgccgc cattgttgct ggcgatagcc
      361 accacttggt ccggggtcag gggagacgct taagactggc cgtcgtttta caacgtcgtg
      421 actgggaaaa catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata
      481 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      541 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
      601 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
      661 atcctgacgg atggcctttt t
//
