LOCUS       sequence-116233-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for expression of cytosolic Grx1-roGFP2 with the Gal4 expression 
            system in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pUAST cyto Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(275..295)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          425..490
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(625..646)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct ctagtcaagg
      121 cactatacat caaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg
      181 tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac
      241 ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag
      301 tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa
      361 acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg
      421 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
      481 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
      541 agaagtaagg ttccttcaca aagatcctct agaggtacct tacttgtaca gctcgtccat
      601 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      661 gttggggtct ttgctcaggg cggagcaggt gctcaggtag tggttgtcgg gcagcagcac
      721 ggggccgtcg ccgat
//
