LOCUS       sequence-116238-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Second generation Rac1 FRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O
  TITLE     SrGAP2-Dependent Integration of Membrane Geometry and 
            Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of 
            Locomotion.
  JOURNAL   Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002.
  PUBMED    26439400
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002."
COMMENT     Sequence Label: pTriEx4-Rac1-2G
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..65)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(72..650)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     primer_bind     complement(669..690)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caccttctga taggcagcct gcacctgagg ttaatcactt agtgatggtg atggtgatgg
       61 tggtgctcga gtcacaacag caggcatttt ctcttcctct tcttcacggg aggcgggcag
      121 aggactgctc ggatcgcttc gtcaaacact gtcttgaggc ctcgctgtgt gagcgccgag
      181 cactccaggt attttacagc accaatctcc ttggccatag ctagaccctg cggataggtg
      241 atgggagtca gcttcttctc cttcagtttc tcgatcgtgt ctttatcatc cctaagatca
      301 agtttagttc ccactaggat gatgggagtg ttgggacagt ggtgccgcac ctcaggatac
      361 cactttgcac ggacattttc aaatgatgca ggactcacaa gggaaaagca aattaagaac
      421 acatctgttt gcggatagga tagggggcgt aatctgtcat aatcttcttg tccagctgta
      481 tcccataagc ccagattcac cggttttcca tctaccataa cattggcaga ataattgtca
      541 aagacagtag ggatatattc tccaggaaat gcattggttg tgtaactgat cagtaggcaa
      601 gttttaccta cagctccgtc tcccaccacc acacacttga tggcctgcat ggtacccccg
      661 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      721 agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      781 ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg
      841 atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg
//
