LOCUS       sequence-116239-         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Second generation Rac1 FRET biosensor for adenovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Fritz RD, Menshykau D, Martin K, Reimann A, Pontelli V, Pertz O
  TITLE     SrGAP2-Dependent Integration of Membrane Geometry and 
            Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of 
            Locomotion.
  JOURNAL   Dev Cell. 2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002.
  PUBMED    26439400
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2015 Sep 29. pii: S1534-5807(15)00582-1. doi: 
            10.1016/j.devcel.2015.09.002."
COMMENT     Sequence Label: pAD/CMV/V5-DEST-Rac1-2G
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..43
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(156..179)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 cgcgggccct ctagatcaac cactttgtac aagaaagctg ggtctagata tctcgagtgc
       61 ggccgctcga tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt
      121 ctgataggca gcctgcacct gaggttaatc acttagtgat ggtgatggtg atggtggtgc
      181 tcgagtcaca acagcaggca ttttctcttc ctcttcttca cgggaggcgg gcagaggact
      241 gctcggatcg cttcgtcaaa cactgtcttg aggcctcgct gtgtgagcgc cgagcactcc
      301 aggtatttta cagcaccaat ctccttggcc atagctagac cctgcggata ggtgatggga
      361 gtcagcttct tctccttcag tttctcgatc gtgtctttat catccctaag atcaagttta
      421 gttcccacta ggatgatggg agtgttggga cagtggtgcc gcacctcagg ataccacttt
      481 gcacggacat tttcaaatga tgcaggactc acaagggaaa agcaaattaa gaacacatct
      541 gtttgcggat aggatagggg gcgtaatctg tcataatctt cttgtccagc tgtatcccat
      601 aagcccagat tcaccggttt tccatctacc ataacattgg
//
