LOCUS       sequence-116241-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pRSET-triple mutation-pa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(21..29,30..47,48..50,51..83,84..86,87..110,111..137,
                     138..194,195..200,201..470,471..488,489..923,924..929,
                     930..1373)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFS
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     407..428
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(534..555)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(795..814)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttaagaagg agatagatct atgggttctc atcatcatca tcatcatggt atggctagca
       61 tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatctcgcca
      121 ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc agagctatag
      181 gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag aacggcatca
      241 aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact
      301 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      361 gccatcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      421 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg
      481 gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      541 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg
      601 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      661 ggcccaccct cgtgaccacc ttcagctacg gcgtgcagtg cttcagccgc taccccgacc
      721 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca
      781 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      841 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      901 tggggcacaa gctggagtac aacctgccgg accaactgac tgaagagcag atcgcagaat
      961 ttaaagagga attctcccta tttgacaagg acggggatgg gacaataaca accaaggagc
     1021 tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg caggacatga
     1081 tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc ctgacaatga
     1141 tggcaagaaa aatgaaatac agggacacgg aagaagaaat tagagaagcg ttcggtgtgt
     1201 ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg atgacaaacc
     1261 ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca gacatcgatg
     1321 gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag tgaaagcttg
     1381 atccggctgc taacaangnc cgaangagtn nntct
//
