LOCUS       sequence-116246-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP with a tdTomato gene from the 
            broadly active CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pATG (pKD-G5)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(185..226)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(290..316)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(323..364)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(428..454)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(600..623)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccggcc gctttacttg tacaagaaag ctgggtctag atatctcgag tgcggccaga
      181 tctgggccat ttgttccatg tgagtgctag taacaggcct tgtgtcctgt gtagttgtac
      241 cagcttgtgt cagtcagtgg ccaaaacaca agctggagta caactacaca gcatacagcc
      301 ttcagcaagc ctccaggatc tgggccattt gttccatgtg agtgctagta acaggccttg
      361 tgtcctgatg aacttcagtc agcttggtca gtcagtggcc aaaaccaagc tgaccctgaa
      421 gttcatcagc atacagcctt cagcaagcct ccaggatcca ctggtcgact cactacctcc
      481 ctttaaacga tcgacggcca cgaagtgctt agcttacttg tacagctcgt ccatgccgta
      541 caggaacagg tggtggcggc cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc
      601 gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag tagccgggca gttgcacggg
      661 cttcttggcc atgtagatgg tcttgaactc caccaggtag tggccgccgt ccttcagctt
      721 cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga
      781 ggcctcccag cccatggtct tcttctgcat tacggg
//
