LOCUS       sequence-116249-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-15 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB3-1
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(424..441)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(424..440)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(433..455)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      682..709
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagacgt ctcgatagcc accacttggt ccggggtcag gccatggtcc tggcacagca
      121 ccggcaacag ccgctgcacc gtttcgagcg cttgcttgcc gccaatgttg ctggcgatag
      181 ccaccacttg gtccggggtc aggccatggt cctggcacag caccggcaac agccgctgca
      241 ccgtttcgag cgcttgcttg ccgccaatgt tgctggcgat agccaccact tggtccgggg
      301 tcaggccatg gtcctggcac agcaccggca acagccgctg caccgtttcg agcgcttgct
      361 tgccgccaat gttgctggcg atagccacca cttggtccgg ggtcaggcca tgagacgctt
      421 aagactggcc gtcgttttac aacgtcgtga ctgggaaaac atccatgcta gcgttaacgc
      481 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
      601 cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
      661 ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
      721 aaactcttcc tggctagcgg tacgcgtatt aattgcgttg cgctcactgc ccgctttcca
      781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatc
//
