LOCUS       sequence-116256-        1471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(30..38,39..56,57..59,60..92,93..95,96..119,120..146,
                     147..203,204..209,210..479,480..497,498..932,933..938,
                     939..1382)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGP
                     VLLPDNHYLSHQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFS
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     416..437
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(543..564)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tagcgctncc ggactcagat ctcgccacca tgggttctca tcatcatcat catcatggta
       61 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
      121 atctcgccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca
      181 gagctatagg tcggctgagc tcactcgaga acgtctatat caccgccgac aagcagaaga
      241 acggcatcaa ggcgaacttc aagatccgcc acaacgtgga ggacggcagc gtgcagctcg
      301 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      361 actacctgag ccatcagtcc gtgctttcga aagaccccaa cgagaagcgc gatcacatgg
      421 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
      481 gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      541 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggtg
      601 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      661 ccgtgccctg gcccaccctc gtgaccacct tcagctacgg cgtgcagtgc ttcagccgct
      721 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      781 aggagcgcac catctccttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt
      841 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      901 gcaacatcct ggggcacaag ctggagtaca acctgccgga ccaactgact gaagagcaga
      961 tcgcagaatt taaagaggaa ttctccctat ttgacaagga cggggatggg acaataacaa
     1021 ccaaggagct ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctgc
     1081 aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc cctgagttcc
     1141 tgacaatgat ggcaagaaaa atgaaataca gggacacgga agaagaaatt agagaagcgt
     1201 tcggtgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt cgccacgtga
     1261 tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc agggaagcag
     1321 acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagt
     1381 gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     1441 ttaaaaaacc tcccacacct ccccctgaac c
//
