LOCUS       sequence-116263-        1372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  short photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: sspa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..90
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     303..324
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(430..451)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gactcagatc tcgccaccat ggtcgactca tcacgtcgta agtggaataa gacaggtcac
       61 gcagtcagag ctataggtcg gctgagctca ctcgagaacg tctatatcac cgccgacaag
      121 cagaagaacg gcatcaaggc gaacttcaag atccgccaca acgtggagga cggcagcgtg
      181 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      241 gacaaccact acctgagcca tcagtccgtg ctttcgaaag accccaacga gaagcgcgat
      301 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      361 tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt caccggggtg
      421 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgaggggc
      481 gagggtgagg gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc
      541 aagctgcccg tgccctggcc caccctcgtg accaccttca gctacggcgt gcagtgcttc
      601 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      661 tacgtccagg agcgcaccat ctccttcaag gacgacggca cctacaagac ccgcgccgag
      721 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      781 gaggacggca acatcctggg gcacaagctg gagtacaacc tgccggacca actgactgaa
      841 gagcagatcg cagaatttaa agaggaattc tccctatttg acaaggacgg ggatgggaca
      901 ataacaacca aggagctggg gacggtgatg cggtctctgg ggcagaaccc cacagaagca
      961 gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat cgacttccct
     1021 gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga agaaattaga
     1081 gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc agagcttcgc
     1141 cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga aatgatcagg
     1201 gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca aatgatgaca
     1261 gcgaagtgag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     1321 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tg
//
