LOCUS       sequence-116265-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-15 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB3-49
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(425..442)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(425..441)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(434..456)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      505..591
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      683..710
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
       61 ggtctagacg tctcgatagc caccacttgg tccggggtca ggccatggtc ctggcacagc
      121 accggcaaca gccgctgcac cgtttcgagc gcttgcttgc cgccaatgtt gctggcgata
      181 gccaccactt ggtccggggt caggccatgg tcctggcaca gcaccggcaa cagccgctgc
      241 accgtttcga gcgcttgctt gccgccaatg ttgctggcga tagccaccac ttggtccggg
      301 gtcaggccat ggtcctggca cagcaccggc aacagccgct gcaccgtttc gagcgcttgc
      361 ttgccgccac cgttgctggc gatagccacc acttggtccg gggtcaggcc atgagacgct
      421 taagactggc cgtcgtttta caacgtcgtg actgggaaaa catccatgct agcgttaacg
      481 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgga agactgggcc
      541 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
      601 gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
      661 actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
      721 caaactcttc ctggctagcg gtacgcgtat taattgcgtt gcgctcactg cccgctttcc
      781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      841 gtttgcgtat tg
//
