LOCUS       sequence-116268-        1383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: spa-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..1383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13..21,22..39,40..42,43..75,76..78,79..102,103..129,
                     130..186,187..192,193..462,463..480,481..915,916..921,
                     922..1365)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYITADKQKNGIKANFHIRHNVEDGSVQLADHYQQNTPIGDGP
                     VLLPDNHYLSHQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFS
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     399..420
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(526..547)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatctcgcca ccatgggttc tcatcatcat catcatcatg gtatggctag catgactggt
       61 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc caccatggtc
      121 gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat aggtcggctg
      181 agctcactcg agaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggcgaac
      241 ttccacatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca ctaccagcag
      301 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag
      361 tccgtgcttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      421 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
      481 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      541 ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg gtgagggcga tgccaccaac
      601 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      661 ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      721 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctcc
      781 ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      841 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      901 aagctggaat acaacctgcc ggaccaactg actgaagagc agatcgcaga atttaaagag
      961 gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg
     1021 gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa
     1081 gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
     1141 aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt gtttgataag
     1201 gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag
     1261 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     1321 caggtaaact acgaagagtt tgtacaaatg atgacagcga agtgagcggc cgcgactcta
     1381 gat
//
