LOCUS       sequence-116270-        1269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  short photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: sspa-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..1269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     285..306
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(412..433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt
       61 cggctgagct cactcgagaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
      121 gcgaacttcc acatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac
      181 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      241 catcagtccg tgctttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      301 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
      361 gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgaggg gcgagggtga gggcgatgcc
      481 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccacctt cagctacggc gtgcagtgct tcagccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      661 atctccttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggaatacaa cctgccggac caactgactg aagagcagat cgcagaattt
      841 aaagaggctt tctccctatt tgacaaggac ggggatggga caataacaac caaggagctg
      901 gggacggtga tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc
      961 aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct gacaatgatg
     1021 gcaagaaaaa tgaaatacag ggacacggaa gaagaaatta gagaagcgtt cggtgtgttt
     1081 gataaggatg gcaatggcta catcagtgca gcagagcttc gccacgtgat gacaaacctt
     1141 ggagagaagt taacagatga agaggttgat gaaatgatca gggaagcaga catcgatggg
     1201 gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagtg agcggccgcg
     1261 actctagat
//
