LOCUS       sequence-116272-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP with a tdTomato gene from the 
            broadly active CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pATK (pKD-G9)
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(352..393)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(457..483)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(490..531)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(595..621)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 caccagccac caccttctga taggcagcct gcacctgagg agtgaattct ttgccaaaat
       61 gatgagacag cacaacaacc agcacgttgc ccaggagctg taggaaagag aagaaggcat
      121 gaacatggtt agcagagggg cccggtttgg actcagagta ttttatcctc atctcaaaca
      181 gtgtatatca ttgtaaccat aaagagaaag gcaggatgat gaccagggtg tagttgtttc
      241 taccaataag aatatttcca cgccagccag aatttatatg cagaaatatt ctaccttatc
      301 atttaattat aacaattctg ggtctagata tctcgagtgc ggccagatct gggccatttg
      361 ttccatgtga gtgctagtaa caggccttgt gtcctgatga acttcagtca gcttggtcag
      421 tcagtggcca aaaccaagct gaccctgaag ttcatcagca tacagccttc agcaagcctc
      481 caggatctgg gccatttgtt ccatgtgagt gctagtaaca ggccttgtgt cctgtgtagt
      541 tgtaccagct tgtgtcagtc agtggccaaa acacaagctg gagtacaact acacagcata
      601 cagccttcag caagcctcca ggatccactg gtcgactcac tagaatgttc tctaaaactg
      661 tgctgaagta caatataata taccctgatt gccttgaaaa aaaagtgatt agagaaagta
      721 cttacaatct gacaaataaa caaaagtgaa ttta
//
