LOCUS       sequence-116279-         374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-KIF1B beta tail 387-1770
FEATURES             Location/Qualifiers
     source          1..374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..325
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 cgcagccccc aagcttggcg cgcctaggcc taccatggct tctagaatcc tctggcatga
       61 gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa
      121 aggcatgttt gaggtgctgg agcccttgca tgctatgatg gaacggggcc cccagactct
      181 gaaggaaaca tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg
      241 caggaagtac atgaaatcag ggaatgtcaa ggacctcctc caagcctggg acctctatta
      301 tcatgtgttc cgacgaatct caaagactag ttatccgtac ggaatgcaga agctgatctc
      361 agaggaggac ctgc
//
