LOCUS       sequence-116289-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicaudal D protein aa1-594 (excluding start Met) with N-terminal 
            tandem dimer Tomato and flag tags and C-terminal FKBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-tdTomato-flag-BicD2 594-FKBP
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(404..427)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(520..543)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tggtgaggac gttgcgcagc tgtttcagct ctgtctgcag ctccagcacc ttccgcacgt
       61 agtactgctc cttggaggcc gactcctgga tcaggctctc ctcccggctc tcaccatcag
      121 cagccacctt cttgtggttt gtatgtgcct ggccaaatgc ctctttgagc tgctccatct
      181 cgctgcggat ggcctcatag tccacctcga gctcctcgaa ctgcagcttg agctggtgct
      241 tctcctccag caccgccagt ccatactcgg ccgcctggat cttctcacgc gtggtctcgg
      301 ccagctcgtg ggacagccgc ttcacctcgg cgcgcagcca ctccggctgc gcctccatca
      361 ccagacgcgc atactcctcc tcctccgacg gcgcggaatg agatttgtca tcgtcatcct
      421 tgtaatccat gatatccatg tacagctcgt ccatgccgta caggaacagg tggtggcggc
      481 cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      541 gcttggtgtc cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg
      601 tcttgaactc caccaggtag cggccgccgt ccttcagctt cagggcctgg tggatct
//
