LOCUS       sequence-116295-        1263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  short photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: sspa-GCaMP6m
FEATURES             Location/Qualifiers
     source          1..1263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..84
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     297..318
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(424..445)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
       61 agagctatag gtcggctgag ctcactcgag aacgtctata tcaccgccga caagcagaag
      121 aacggcatca aggcgaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc
      181 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      241 cactacctga gccatcagtc cgtgctttcg aaagacccca acgagaagcg cgatcacatg
      301 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      361 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      421 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag gggcgagggt
      481 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      541 cccgtgccct ggcccaccct cgtgaccacc ttcagctacg gcgtgcagtg cttcagccgc
      601 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      661 caggagcgca ccatctcctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag
      721 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      781 ggcaacatcc tggggcacaa gctggaatac aacctgccgg accaactgac tgaagagcag
      841 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
      901 accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg
      961 caggacatga tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc
     1021 ctgacaatga tggcaagaaa agggagctac agggacacgg aagaagaaat tagagaagcg
     1081 ttcggtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg
     1141 atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca
     1201 gacatcgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag
     1261 tga
//
