LOCUS       sequence-116297-        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: spa-GCaMP6m
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..9,10..27,28..30,31..63,64..66,67..90,91..117,
                     118..174,175..180,181..450,451..468,469..903,904..909,
                     910..1353)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6m
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGP
                     VLLPDNHYLSHQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFS
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKGSYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     387..408
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(514..535)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
       61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
      121 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag
      181 aacgtctata tcaccgccga caagcagaag aacggcatca aggcgaactt caagatccgc
      241 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgtgctttcg
      361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      421 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
      481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      541 ggccacaagt tcagcgtgag gggcgagggt gagggcgatg ccaccaacgg caagctgacc
      601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      661 ttcagctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      721 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatctcctt caaggacgac
      781 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggaatac
      901 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
      961 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     1021 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1081 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa agggagctac
     1141 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     1201 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     1261 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     1321 gaagagtttg tacaaatgat gacagcgaag tga
//
