LOCUS       sequence-116298-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of HA tagged ubiquitin K29R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Lim KL, Chew KC, Tan JM, Wang C, Chung KK, Zhang Y, Tanaka Y, Smith 
            W, Engelender S, Ross CA, Dawson VL, Dawson TM
  TITLE     Parkin mediates nonclassical, proteasomal-independent ubiquitination
            of synphilin-1: implications for Lewy body formation.
  JOURNAL   J Neurosci. 2005 Feb 23. 25(8):2002-9.
  PUBMED    15728840
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2005 Feb 23. 25(8):2002-9."
COMMENT     Sequence Label: pRK5-HA-Ubiquitin-K29R
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..106
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      398..407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    411..545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(448..467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     502..521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        614..805
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(637..657)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 actgcacctc ggttctatcg attgaattcc cgaaccgaca gtcggtctct tcaccaaggc
       61 cattcgcgcc accatgggct acccctatga tgtgcctgac tacgcagatc tcaatggtgg
      121 tggtggtggg tcgaccatgc agatcttcaa gacgttaacc ggtaaaacca taactctaga
      181 agttgaacca tccgatacca tcgaaaacgt taaggctagg attcaagaca aggaaggcat
      241 tccacctgat caacaaagat tgatctttgc cggtaagcag ctcgaggacg gtagaacgct
      301 gtctgattac aacattcaga aggagtccac cctgcacctg gtcctccgtc tcagaggtgg
      361 ttgagcggcc gctaagtaag taaggatccc cagcttggcc gccatggccc aacttgttta
      421 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      481 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      541 ggatcgggaa ttaattcggc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
      601 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
      661 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      721 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      781 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      841 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      901 aggccgcctc ggcctctgag ctattccaga a
//
