LOCUS       sequence-116306-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-15 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB3-35
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(423..440)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(423..439)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(432..454)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      503..589
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      681..708
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgta cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg
       61 tctagacgtc tcgatagcca ccacttggtc cggggtcagg ccatggtcct ggcacagcac
      121 cggcaacagc cgctgcaccg tttcgagcgc ttgcttgccg ccattgttgc tggcgatagc
      181 caccacttgg tccggggtca ggccatggtc ctggcacagc accggcaaca gccgctgcac
      241 cgtttcgagc gcttgcttgc cgccaatgtt gctggcgata gccaccactt ggtccggggt
      301 caggccatgg tcctggcaca gcaccggcaa cagccgctgc accgtttcga gcgcttgctt
      361 gccgccattg ttgctggcga tagccaccac ttggtccggg gtcaggccat gagacgctta
      421 agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag cgttaacgcg
      481 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt
      541 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
      601 ggatttgaac gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
      661 tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
      721 aactcttcct ggctagcggt acgcgtatta attgcgttgc gctcactgcc cgctttccag
      781 tc
//
