LOCUS       sequence-116308-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Zyxin-CNL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pCNL-N1_zyxin
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..131
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
       61 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      121 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
      181 gcgaccggtg gatcccgggc ccgcggtacc gtcgacgggg tctgggctct agcggagtgg
      241 cacttccgac aaaggacgtg gccatccaga gggaaacagc cgttgtcatc tgcctcaatg
      301 gacagaggtt tcccacagtc ctcacacttg taacacttca tatgaaagtt cttatccagc
      361 gccactactc gcacagtctc gtctcggcca ggctcaggca tgattggctc cgagcagacg
      421 gagcaccttg gagcgtattg cttgtgatag tcagggacac agtggggctg attggcctgg
      481 tccacaatga aggaggtgcc ctccagggga caggcgcaga ccacacaggt gaagcactgt
      541 gggtggtagg ctttgccagt ggccctcagc atgcggtcag tgatgggctg cccacaggtg
      601 ttgcacttct ccaaagtgtc ggtgtagcag ccctcacaat atggtgctcc ctccaggcta
      661 tagaactgct gtccctgcag ctgctgctga cactgatggc aagtgaagca ggtgatgtgg
      721 aacagttgtc ccagtgcacg aaccgcaggc tgtgcacggg ccagtggctg attgcatttg
      781 ccacaggact cattcactgc cacgctctgc ctctgagggt gttccatgtc ctgcatcagc
      841 tgctgggtca gctgctccaa ctcctctacc tccttcaggg tcaaggggcc tgggcctcca
      901 ggagagcgta cctggttttg gttttgagct ggtggaggct gtgggtgctg cttctcttga
      961 actaggggct tctccttctg ctgagcatag gtgaagctag ggg
//
