LOCUS       sequence-116314-        1416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: pRSET-spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(34..42,43..60,61..63,64..96,97..99,100..123,124..150,
                     151..207,208..213,214..483,484..501,502..936,937..942,
                     943..1386)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGP
                     VLLPDNHYLSHQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFS
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      130..139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     420..441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(547..568)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagataga tctatgggtt ctcatcatca tcatcatcat
       61 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      121 aaggatctcg ccaccatggt cgactcatca cgtcgtaagt ggaataagac aggtcacgca
      181 gtcagagcta taggtcggct gagctcactc gagaacgtct atatcaccgc cgacaagcag
      241 aagaacggca tcaaggcgaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag
      301 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      361 aaccactacc tgagccatca gtccgtgctt tcgaaagacc ccaacgagaa gcgcgatcac
      421 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      481 aagggcggta ccggagggag catggtgagc aagggcgagg agctgttcac cggggtggtg
      541 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gaggggcgag
      601 ggtgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
      661 ctgcccgtgc cctggcccac cctcgtgacc accttcagct acggcgtgca gtgcttcagc
      721 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      781 gtccaggagc gcaccatctc cttcaaggac gacggcacct acaagacccg cgccgaggtg
      841 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      901 gacggcaaca tcctggggca caagctggag tacaacctgc cggaccaact gactgaagag
      961 cagatcgcag aatttaaaga ggaattctcc ctatttgaca aggacgggga tgggacaata
     1021 acaaccaagg agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag
     1081 ctgcaggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag
     1141 ttcctgacaa tgatggcaag aaaaatgaaa tacagggaca cggaagaaga aattagagaa
     1201 gcgttcggtg tgtttgataa ggatggcaat ggctacatca gtgcagcaga gcttcgccac
     1261 gtgatgacaa accttggaga gaagttaaca gatgaagagg ttgatgaaat gatcagggaa
     1321 gcagacatcg atggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg
     1381 aagtgaaagc ttgatccggc tgctaacaaa gcccga
//
