LOCUS       sequence-116317-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of tdTomato from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pART (pT-2)
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(140..163)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(338..357)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     607..625
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcaagggtcc ccaaactcac cctgaagttc tcaggatcct tcgaatctag actttacttg
       61 tacagctcgt ccatgccgta caggaacagg tggtggcggc cctcggagcg ctcgtactgt
      121 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag
      181 tagccgggca gttgcacggg cttcttggcc atgtagatgg tcttgaactc caccaggtag
      241 tggccgccgt ccttcagctt cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg
      301 gggtacaggc gctcggtgga ggcctcccag cccatggtct tcttctgcat tacggggccg
      361 tcggggggga agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc gtcctgcagg
      421 gaggagtcct gggtcacggt caccagaccg ccgtcctcga agttcatcac gcgctcccac
      481 ttgaagccct cggggaagga cagcttcttg taatcgggga tgtcggcggg gtgcttcacg
      541 tacgccttgg agccgtacat gaactggggg gacaggatgt cccaggcgaa gggcaggggg
      601 ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
      661 tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgcgcac cttgaagcgc
      721 atgaactctt tgatgacggc catgttgttg tcctcggagg aggcggtgcc ggagctgccg
      781 ctgccggtgc tgccggtgcc atgcccca
//
