LOCUS       sequence-116328-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta-tubulin-CNL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pCNL-N1_TUBB5
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     387..408
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      450..459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gacgttgtcg ctaaagtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
       61 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      121 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      181 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      241 gcactgcacg ccccaggaca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      301 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      361 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      421 cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat cccgggcccg
      481 cggtaccgtc gactgggcct cctcttctgc ctcctctccg aaatcctctt cctcttccgc
      541 ggtggcatcc tggtactgct ggtactcaga gacgaggtcg ttcatgttgc tctcagcttc
      601 ggtgaactcc atctcgtcca tgccctcacc cgtataccag tggaggaaag ccttccggcg
      661 gaacatagcc gtaaactgct cagagatgcg cttgaagagc tcctggatgg ctgtgctgtt
      721 tccaatgaag gtgactgcca tcttgaggcc acgcggtggg atgtcacaga cagctgtctt
      781 gacattgttg gggatccatt ccacgaagta gctgctattc ttgttctgca cgttgagcat
      841 ctgctcatcc acctccttca tggacatccg tccacggaag acggcggcaa ctgtgaggta
      901 ccggccgtgg cgcgggtcgc aggcggccat catgttcttg gcatcgaaga cctgctgggt
      961 aagttcaggc acagtgagg
//
