LOCUS       sequence-116331-        6675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene expression in plants. A promoter and/or reporter can be cloned 
            into this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-4"; volume: "9(6):e98988. doi"; pages: " 
            10.1371/journal.pone.0098988. eCollection 2014"
COMMENT     Sequence Label: pKDH
FEATURES             Location/Qualifiers
     source          1..6675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    66..90
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      287..928
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        1017..1196
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1218..2246
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KK"
     misc_feature    2648..2672
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2787..3375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2867..2886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3546..4337,4338..4406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4169..4188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4407..4511)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4552..5162
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4639..5170
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gcgcgcggta cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
       61 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
      121 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccactagac cagtaccttg
      181 gaggcctgtg cgcatcccag ggtgggtccc gtataccccg gggaattcaa gcttggatcc
      241 ctcgaggtta acacgcgtgt cgacgagctc ctgcaggatc ctctagagct ttcgttcgta
      301 tcatcggttt cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg cacacaccag
      361 aatcctactg agttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt
      421 gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat
      481 ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt
      541 tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt
      601 tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt
      661 aaaacacttg tagttgtacc attatgctta ttcactaggc aacaaatata ttttcagacc
      721 tagaaaagct gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg
      781 aactttcctt tatgtaattt tccagaatcc ttgtcagatt ctaatcattg ctttataatt
      841 atagttatac tcatggattt gtagttgagt atgaaaatat tttttaatgc attttatgac
      901 ttgccaattg attgacaaca tgcatcaatc gatgatcatg agcggagaat taagggagtc
      961 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
     1021 cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata
     1081 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
     1141 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     1201 ctcatattca ctctcaaatg ggaaagaagc cagagcttac tgctacttcc gtggagaagt
     1261 tccttattga gaagttcgat tccgtgtctg atcttatgca actttctgag ggtgaagagt
     1321 ctagggcttt ctcttttgat gttggtggaa ggggttatgt tcttagggtg aactcttgcg
     1381 ctgatggatt ctacaaggat cgttacgttt acaggcattt cgcttcagct gctttgccaa
     1441 ttccagaggt gttggatatt ggagagttct ctgagtctct tacttactgc atttctaggc
     1501 gtgctcaagg tgttactctt caggatcttc cagaaactga gcttccagct gttcttcaac
     1561 cagttgctga ggctatggat gctattgctg ctgctgatct ttctcaaact tccggatttg
     1621 gaccatttgg tccacaaggt atcggacagt acactacttg gagggatttc atttgcgcta
     1681 ttgctgatcc acatgtttac cactggcaga ctgtgatgga tgatactgtt tctgcttctg
     1741 ttgctcaggc tcttgatgaa cttatgcttt gggctgagga ttgtccagaa gttaggcacc
     1801 ttgttcacgc tgatttcgga tctaacaacg tgctcactga taacggaagg atcactgctg
     1861 ttattgattg gtccgaggct atgtttggag attctcagta cgaggtggca aacatttttt
     1921 tctggcgacc ttggcttgct tgtatggaac agcagactag atatttcgag agaaggcatc
     1981 cagaacttgc tggatctcca aggcttaggg cttacatgct taggattgga cttgatcagc
     2041 tttaccagtc tctcgtggat ggaaattttg atgatgctgc ttgggctcag ggaagatgtg
     2101 atgctattgt gaggtctggt gctggaactg ttggaaggac tcagattgct agaagatcag
     2161 ctgctgtttg gactgatgga tgcgttgaag tgcttgctga ttctggaaat agaaggccat
     2221 ctactagacc aagggctaag aagtgaatcg ttcaaacatt tggcaataaa gtttcttaag
     2281 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     2341 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     2401 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagctcg caaactagga
     2461 taaattatcg ctcgcggtgt catctatgtt actagtagat ctggtgaccc ctatgtaagg
     2521 ggccggccat ttaaattcgc gattaattaa cctgtggttg gcatgcacat acaaatggac
     2581 gaacggataa accttttcac gcccttttaa atatccgatt attctaataa acgctctttt
     2641 ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg
     2701 aaacgacaat ctgatatcgc gcgcggtacc tgagcaaaag gccagcaaaa ggccaggaac
     2761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3421 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3541 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3661 ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca
     3721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3841 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4141 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4441 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4501 ggggttccgc gcacatttcc ccgaaaagtg ccacgggccc cgctcaccgg gctggttgcc
     4561 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
     4621 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     4681 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     4741 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     4801 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     4861 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     4921 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     4981 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     5041 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     5101 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     5161 ccggcccgct gtcgactacc tagatgtggc gcaacgatgc cggcgacaag caggagcgca
     5221 ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg
     5281 gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg
     5341 gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg
     5401 caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc
     5461 cgcaaggagg gtgaatgaat cgcaccttcg atcgcaaggc gtatcgccag gaactgatcg
     5521 acgccggctt ctcggccgaa gatgccgaaa cgatcgcctc gcgcaccgtc atgcgtgccc
     5581 cgcgcgaaac cttccagtcg gtcggctcga tggtccagca ggccaccgcc aagatcgaac
     5641 gcgattcggt ccagctggcc ccgccggccc ttccggcccc gtcggccgcc gtcgaacgct
     5701 cgcgccggct ggaacaggaa gccgccggcc tggccaagtc gatgaccatc gacacccgcg
     5761 gcaccatgac caccaagaag cgcaagaccg ccggcgagga tctggccaag caggtcagcg
     5821 aagccaagca ggccgccctg ctgaagcata ccaagcagca gatcaaggaa atgcagctgt
     5881 cgctgttcga catcgccccg tggccggata ccatgcgggc catgccgaat gataccgccc
     5941 gctcggccct gttcaccacc cgcaataaga agatcccgcg cgaagccctc cagaataagg
     6001 tcatcttcca cgtcaacaag gatgtcaaga tcacctatac cggcgtcgaa ctgcgggccg
     6061 atgatgatga actggtctgg cagcaggtcc tggaatatgc caagcgcacc ccgatcggcg
     6121 aaccgatcac cttcaccttc tatgaactgt gccaggatct gggctggtcg atcaatggcc
     6181 gctattatac caaggccgaa gaatgcctgt cgcgcctcca ggcgaccgcg atgggcttca
     6241 cctcggatcg cgtcggccat ctggaatcgg tcagcctgct gcaccgcttc cgcgtcctgg
     6301 atcgcggcaa gaagacctcg cgctgccagg tcctgatcga cgaagaaatc gtcgtcctgt
     6361 tcgccggcga tcattatacc aagttcatct gggagaagta tcgcaagctg tcgccgaccg
     6421 cccgccgcat gttcgattat ttctcgtcgc accgcgaacc gtatccgctg aagctggaaa
     6481 ccttccgcct gatgtgcggc tcggattcga cccgcgtcaa gaagtggcgc gaacaggtcg
     6541 gcgaagcctg cgaagaactg cgcggctcgg gcctggtcga acatgcctgg gtcaatgatg
     6601 atctggtcca ttgcaagcgc tagggccttg tggggtcagt tccggctggg ggttcagcca
     6661 gcgctttacg atatc
//
