LOCUS       sequence-116335-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Golgi-ONL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pONL-N1_Golgi
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      306..315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             312..965
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             312..965
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatgagg cttcgggagc
       61 cgctcctgag cggcagcgcc gcgatgccag gcgcgtccct acagcgggcc tgccgcctgc
      121 tcgtggccgt ctgcgctctg caccttggcg tcaccctcgt ttactacctg gctggccgcg
      181 acctgagccg cctgccccaa ctggtcggag tctccacacc gctgcagggc ggctcgaaca
      241 gtgccgccgc catcgggcag tcctccgggg agctccggac cggaggggcc aaggatccac
      301 cggtcgccac catggtgagc gtgatcaagc ccgagatgaa gatgaggtac tacatggacg
      361 gctccgtcaa tgggcatgag ttcacaatcg agggtgaggg cacaggcaga ccttacgagg
      421 gacatcagga gatgacactg cgcgtcacaa tggccgaggg cgggccaatg cctttcgcct
      481 tcgacctggt gtcccacgtg ttctgttacg gccacagagt ttttactaaa tatccagaag
      541 agatcccaga ctatttcaag caggcctttc ctgagggcct gtcctgggag aggtccctgg
      601 agttcgagga cggcggctcc gcctccgtga gcgcccacat cagcctgagg ggcaacacct
      661 tctaccacaa gtccaagttc accggcgtga acttccccgc cgacggcccc atcatgcaga
      721 accagagcgt ggactgggag ccctccaccg agaagatcac cgccagcgac ggcgtgctga
      781 agggcgacgt gaccatgtac ctgaagctgg agggcggcgg caaccacaag tgccagatga
      841 agaccaccta caaggccgcc aaggagatcc tggagatgcc cggcgaccac tacatcggcc
      901 acaggctggt gaggaagacc gagggcaaca tcaccgagca ggtggaggac gccgtggccc
      961 actccggtac caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt
     1021 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg
     1081 a
//
