LOCUS       sequence-116353-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inverted GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: iGFP
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     53..77
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     184..200
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     185..201
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(234..267)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(314..347)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(314..347)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(405..426)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     754..773
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
       61 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      121 accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt accgggcccc
      181 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc gggataactt
      241 cgtatagtac acattatacg aagttatggc taagctttgt gccatgccat tgatgctagc
      301 acagcctgaa tggataactt cgtataggat accttatacg aagttatacc ggtgctagct
      361 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      421 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
      481 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      541 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcgc cttgatgccg
      601 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      661 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      721 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttg
//
