LOCUS       sequence-116355-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inverted GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: iGFP
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..32
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16..32
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(34..67)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(34..67)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(82..115)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(147..149,150..152,153..665)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(192..213)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(453..472)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gactcgagcg gccgctctag aactagtgga tccataactt cgtataggat accttatacg
       61 aagttatctc agctagaagc cataacttcg tatagtacac attatacgaa gttatgttta
      121 ctactacatg ttactggcgc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      181 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtctg
      241 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      301 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      361 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      421 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      481 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      541 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      601 atatcatggc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc cgccacaaca
      661 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      721 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agc
//
