LOCUS       sequence-116357-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector: Neuroligin 1 shRNA under control of 
            mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Chih B, Engelman H, Scheiffele P
  TITLE     Control of excitatory and inhibitory synapse formation by 
            neuroligins.
  JOURNAL   Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27.
  PUBMED    15681343
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-02-25"; volume: "307(5713)"; pages: "1324-8. Epub 2005 
            Jan 27"
COMMENT     Sequence Label: pLenLox_U6 Nlgn1
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(5..7,8..10,11..523)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(50..71)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(311..330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     658..679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
       61 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      121 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      181 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      241 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      301 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      361 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      421 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      481 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      541 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      601 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      661 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
//
