LOCUS       sequence-116396-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_1_111
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(396..428)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             436..458
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(489..508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(490..508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggtggtggt ggtggtgctc gagtgcggcc gctacgagtt tgttcttggc catcttcgtg
       61 tttgccttag caaactctag tcgtagtgtt tgcggaattt caggatcgaa gcggatgcca
      121 ttcaaagcat tctttgcagc ctctgcttct gagcgactgt caaaactgac aaaacctaca
      181 ggctgtttag atgtgagctt tataagagaa ccctcatagc ccttaaatgg tctgaaaagc
      241 agatagagct cccgaggttt gatatccaga ggaaggccac tgacaaatag ggtccggacc
      301 tcctcctcct gaaggttggc ctcgctcggg gtgttctcct tctcggcttt gccgccgttg
      361 ttcatgggtc gacggagctc gaattcggat ccgcgaccca tttgctgtcc accagtcatg
      421 ctagccatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt
      481 tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg ggcagcgttg
      541 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
      601 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
      661 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
      721 cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat t
//
