LOCUS       sequence-116398-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAGHA-tagged RNAsH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     RNAseH1 plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RNAseH1
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..79)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    132..156
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggaatcagt tatctatgcg gccgcggatc aggtacgcgt agaatcgaga ccgaggagag
       61 ggttagggat aggcttacct tcgaaccgcg ggccctctag acttgagcgg ccgccactgt
      121 gctggatatc aaccactttg tacaagaaag ctgggtctag atatctcgag tgcggccgct
      181 cagtcttccg attgtttagc tccttctctg gctaatctgt cagcttcttc attgcctata
      241 aatcccgaat gaccaggaac atgcatccac tgaatgtcca tcccctgggt aagcctctcc
      301 agtgccacaa agtcctcttt gttgatcacc tctttccctg cacttgtctt ccacccattt
      361 ttcttccaac cttgaaccca gttagttata ccatttatcg taaacatact gtctgtatac
      421 agaaccagtt tattgatgtt ttgagtcttt gcttgttcaa tggctttgca ggctgcatga
      481 atttccgctc tttggtttgt ctgccgccca ggaagtctaa tgcctacatt taaaggatgg
      541 cccggccccc agtaaacgcc gattcctgct cgcggccttc tacgcccatt actggagcag
      601 cagccatcag tgtagacgac gacgaagtct cccatgtagg aaaacgtgtc tctgctaact
      661 ggaggcgccg gctccacgct cggcttcatg tgctttgcat acggctctgc gctttcatgt
      721 ccatctccat ccagtggctc acggagtcgc ttgctggctt tcgcctccga ttcttgtcca
      781 tgttgatttt catgcccttc tgaaacttcc gggcttgcag attt
//
