LOCUS       sequence-116399-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     RNAseH1 plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-DestFLAGHA_RNAseH1
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      129..138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             138..161
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             162..188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    204..228
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgagctcg gatcgatcca tcgatagtgt ggtggaattc
      121 tgatcgccgc cgccatggac tacaaggacg acgatgacaa gtacccttat gacgtgcccg
      181 attacgcttt gaaccagata tcaacaagtt tgtacaaaaa agcaggctcc accatgggaa
      241 ccaattcagt cgacatgagc tggcttctgt tcctggccca cagagtcgcc ttggccgcct
      301 tgccctgccg ccgcggctct cgcgggttcg ggatgttcta tgccgtgagg aggggccgca
      361 agaccggggt ctttctgacc tggaatgagt gcagagcaca ggtggaccgg tttcctgctg
      421 ccagatttaa gaagtttgcc acagaggatg aggcctgggc ctttgtcagg aaatctgcaa
      481 gcccggaagt ttcagaaggg catgaaaatc aacatggaca agaatcggag gcgaaagcca
      541 gcaagcgact ccgtgagcca ctggatggag atggacatga aagcgcagag ccgtatgcaa
      601 agcacatgaa gccgagcgtg gagccggcgc ctccagttag cagagacacg ttttcctaca
      661 tgggagactt cgtcgtcgtc tacactgatg gctgctgctc cagtaatggg cgtagaaggc
      721 cgcgagcagg aatcggcgtt tactgggggc cgggccatcc tttaaatgta ggcattagac
      781 ttcctgggcg gcagacaaac caaagagcgg aaattcatgc agcctgcaaa gccattgaac
      841 aagcaaagac tcaaaacatc aataaactgg ttctgtatac a
//
