LOCUS       sequence-116413-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_1_129
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(465..497)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             505..527
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(558..577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(559..577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcggc aatgaactga
       61 ggtacagtgt tgggcagagg agtactgggg tttggagtcc ctacgagttt gttcttggcc
      121 atcttcgtgt ttgccttagc aaactctagt cgtagtgttt gcggaatttc aggatcgaag
      181 cggatgccat tcaaagcatt ctttgcagcc tctgcttctg agcgactgtc aaaactgaca
      241 aaacctacag gctgtttaga tgtgagcttt ataagagaac cctcatagcc cttaaatggt
      301 ctgaaaagca gatagagctc ccgaggtttg atatccagag gaaggccact gacaaatagg
      361 gtccggacct cctcctcctg aaggttggcc tcgctcgggg tgttctcctt ctcggctttg
      421 ccgccgttgt tcatgggtcg acggagctcg aattcggatc cgcgacccat ttgctgtcca
      481 ccagtcatgc tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      541 ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctcgg
      601 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
      661 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
      721 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
      781 t
//
