LOCUS       sequence-116421-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the mutated N terminal part (R5D-K5D N23, 
            transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein 
            (C-ter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..708,709..711,712..714))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(38..58)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     653..681
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    725..749
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa gaaggaccat
       61 gtggtcacgc ttttcgttgg gatctttcga aagggcagat tgtgtcgaca ggtaatggtt
      121 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
      181 ttgaacggat ccatcttcaa tgttgtggcg aattttgaag ttagctttga ttccattctt
      241 ttgtttgtct gccgtgatgt atacattgtg tgagttatag ttgtactcga gtttgtgtcc
      301 gagaatgttt ccatcttctt taaaatcaat accttttaac tcgatacgat taacaagggt
      361 atcaccttca aacttgactt cagcacgcgt cttgtagttc ccgtcatctt tgaaagatat
      421 agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gccgtttcat
      481 atgatccgga taacgggaaa agcattgaac accataagag aaagtagtga caagtgttgg
      541 ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagct ttccgtatgt
      601 agcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa
      661 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttgctag ccattctaga
      721 tcgaaccact ttgtacaaga aagctgggtc ggcgcgccca cccttcgcgt agcccacgcc
      781 agcccctgcg cccagcattg cgactgagat cagcatgtcg t
//
