LOCUS       sequence-116429-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C terminally V5-tagged C11orf83/UQCC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA3.2-C11orf83-V5
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             131..151
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(456..480)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             506..547
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(587..606)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    631..679
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctc
      121 cgcggccgcc ttgtttaact ttaagaagga gcccttcacc atggattcct tgcggaaaat
      181 gctgatctca gtcgcaatgc tgggcgcagg ggctggcgtg ggctacgcgc tcctcgttat
      241 cgtgaccccg ggagagcggc ggaagcagga aatgctaaag gagatgccac tgcaggaccc
      301 aaggagcagg gaggaggcgg ccaggaccca gcagctattg ctggccactc tgcaggaggc
      361 agcgaccacg caggagaacg tggcctggag gaagaactgg atggttggcg gcgaaggcgg
      421 cgccggcggg aggtcaccga agggtgggcg cgccgaccca gctttcttgt acaaagtggt
      481 tgatctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
      541 ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa acacggaagg
      601 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
      661 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
      721 accccaccga gaccccattg gggccaatac gcccgcg
//
