LOCUS       sequence-116430-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C terminally V5-tagged C11orf83/UQCC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA3.2-C11orf83-V5
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    54..102
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     127..146
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(186..227)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    253..277
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             582..602
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(614..638)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(667..686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(731..782)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(760..780)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
       61 cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt
      121 ccggtattgt ctccttccgt gtttcagtta gcctcccccg tttaaactca ttactaaccg
      181 gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc gaaccgcggg
      241 ccctctagat caaccacttt gtacaagaaa gctgggtcgg cgcgcccacc cttcggtgac
      301 ctcccgccgg cgccgccttc gccgccaacc atccagttct tcctccaggc cacgttctcc
      361 tgcgtggtcg ctgcctcctg cagagtggcc agcaatagct gctgggtcct ggccgcctcc
      421 tccctgctcc ttgggtcctg cagtggcatc tcctttagca tttcctgctt ccgccgctct
      481 cccggggtca cgataacgag gagcgcgtag cccacgccag cccctgcgcc cagcattgcg
      541 actgagatca gcattttccg caaggaatcc atggtgaagg gctccttctt aaagttaaac
      601 aaggcggccg cggagcctgc ttttttgtac aaacttgttg atagcttaac tagccagctt
      661 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
      721 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      781 tcaat
//
