LOCUS       sequence-116432-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible expression of mutant IDH2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes 
            NI, Feist AM, Vander Heiden MG, Metallo CM
  TITLE     Tracing compartmentalized NADPH metabolism in the cytosol and 
            mitochondria of mammalian cells.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):253-63. doi: 
            10.1016/j.molcel.2014.05.008. Epub 2014 May 29.
  PUBMED    24882210
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.05.008"; journalName: "Mol Cell"; date: 
            "2014-07-17- 17"; volume: "55"; issue: "2"; pages: "253-63"
COMMENT     Sequence Label: pSLIK-IDH2-R172K-FLAG
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    139..163
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(184..249)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 acgctcgctg cgcccttcgt ctgacgtggc agcgctcgcc gtgaggaggg gggcgcccgc
       61 gggaggcgcc aaaacccggc gcggaggcca gatcttgggt gggttaatta accatcttta
      121 tggctcgaga tgttatcaac cactttgtac aagaaagctg ggtctagata tctcgagtca
      181 ctacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac cgtcatggtc
      241 tttgtagtca gcccgggatc ctctagaata ctgcctgccc agggctctgt ccaggttgct
      301 cttgatggtg tcgaggaagt ccgtggtgtt caggaagtgc tcgttcagct tcacattgct
      361 gaggccgtga atgcagcccg ccaggtcctt ggtcatggct ccactctcca ccgtctccac
      421 gcacaccttc tccagcatct gggcaaacct gatgaggtct tggttcccat ccagcttccc
      481 ccggtgctcc aggccacgtg tccaggcaaa gatgctggcg atggggttgg tgctggtggg
      541 ccggcccttc tggtgctccc gatagtggcg ggtgacggtc ccatgagcgg cctcagcctc
      601 aatcgtcttc ccatcagggc agaccaggac ggacgtcatc agg
//
