LOCUS       sequence-116433-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_20_196
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(612..644)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             652..674
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcgca gaactgacgg
       61 gacttccagc cctgagaagt agcctcggag ggagggagcc agcgcatctg ggcatgcagt
      121 gaagcgggat aggtgaaagc aggaggaggt agagcaggcg ctaactccgc tgggtacaga
      181 gggtacgggg cccacacttc agggctactg gggtaaagtg caggcactgt gagctcatat
      241 ggctctctgg caatgaactg aggtacagtg ttgggcagag gagtactggg gtttggagtc
      301 cctacgagtt tgttcttggc catcttcgtg tttgccttag caaactctag tcgtagtgtt
      361 tgcggaattt caggatcgaa gcggatgcca ttcaaagcat tctttgcagc ctctgcttct
      421 gagcgactgt caaaactgac aaaacctaca ggctgtttag atgtgagctt tataagagaa
      481 ccctcatagc ccttaaatgg tctgaaaagc agatagagct cccgaggttt gatatccaga
      541 ggaaggccac tgacaaatag ggtccggacc tcctcctcca tgggtcgacg gagctcgaat
      601 tcggatccgc gacccatttg ctgtccacca gtcatgctag ccatatgtat atctccttct
      661 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
      721 attaatttcg cgggatcgag atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
      781 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact gg
//
