LOCUS       sequence-116435-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_1_101
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(382..414)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             422..444
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(475..494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(476..494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg ccttagcaaa
       61 ctctagtcgt agtgtttgcg gaatttcagg atcgaagcgg atgccattca aagcattctt
      121 tgcagcctct gcttctgagc gactgtcaaa actgacaaaa cctacaggct gtttagatgt
      181 gagctttata agagaaccct catagccctt aaatggtctg aaaagcagat agagctcccg
      241 aggtttgata tccagaggaa ggccactgac aaatagggtc cggacctcct cctcctgaag
      301 gttggcctcg ctcggggtgt tctccttctc ggctttgccg ccgttgttca tgggtcgacg
      361 gagctcgaat tcggatccgc gacccatttg ctgtccacca gtcatgctag ccatatgtat
      421 atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat
      481 agtgagtcgt attaatttcg cgggatcgag atctcgggca gcgttgggtc ctggccacgg
      541 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
      601 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
      661 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
      721 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
      781 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
      841 tttctctggt cccgccgcat ccataccgcc agttgtttac cc
//
