LOCUS       sequence-116456-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note:
            This is being tested..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC94-pmax-dCas9VP160-2A-puro
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..457)
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(491..511)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcgggatct ctcagntcac cgcatgntta gaaggcttcc tctgccctct ccgctgccag
       61 atctatcgnt atncaacata tccaaatcga agtcatcgag cgcatcggag cccagcatgt
      121 ccaggtcaaa gtcgtccagg gcatcactcc ccagcatatc caagtcaaaa tcatccaagg
      181 catcggaccc cagcatgtcc agatcgaaat cgtccagggc gtcgctgccg agcatgtcca
      241 ggtcgaaatc atcaagggca tcgcttccca acatatcaag gtcgaaatca tccagtgcgt
      301 ctgaccctaa catatcgaga tcgaaatcgt ccagagcatc ggagccgagc atgtccagat
      361 caaagtcatc caatgcgtcg cttcccaaca tatccaggtc aaagtcatcg agggcatcag
      421 aacccagcat gtcgagatcg aaatcgtcca gcgcgtcggc gcgcccggat ccggccggcc
      481 cgctggcctc cacctttctc ttcttcttgg ggctgtcgcc tcccagctga gacaggtcga
      541 tccgtgtctc gtacaggccg gtgatgctct ggtggatcag gntggcgtcc agcanctctt
      601 tggtgctggt gtacctcttc cggtcgatgg tggtgtcnaa gtacttgaag gcggcagggg
      661 ctccnanatt ggtcagggtn aaacaggtgg atgatantct cggcctgctc tctgatgggc
      721 ttatcccgag tnnntgntgt angaggacaa ncactttgtc ctaaatgagc ntnagcccan
      781 gaatnnantc tntttggana aantcgactg gatctngacc cgnangnaat cctccctcaa
      841 anggtaaann gcgttgnngc atggttncca acnaaacnan cntnttttta tggctccaat
      901 ttatcccnna gggaggggaa gnccnnnttt nagcatgtnc tnaaangaan gggaatggnc
      961 naagtgannn ancgnaaagn tttctaacnt nantttgaga ngngacnnta ggngncacta
     1021 antcnnnatn ttcaccatnt nactgatgta nttnaccncg gncntannaa ncnagacaag
     1081 ntgattannn nttnttctcn ggagnaaann tattttctaa gganttnna
//
