LOCUS       sequence-116457-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note:
            This is being tested..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC94-pmax-dCas9VP160-2A-puro
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             51..71
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattcaccgg tgccaccatg tacccatacg atgttccaga ttacgcttcg ccgaagaaaa
       61 agcgcaaggt cgaagcgtcc gacaagaagt acagcatcgg cctggccatc ggcaccaact
      121 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      181 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      241 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
      301 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
      361 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
      421 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
      481 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
      541 atctggccct ggcccac
//
