LOCUS       sequence-116460-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+D839A+H840A+N863A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt
       61 cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctag ctttatcgga
      121 tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg gcacgatagc
      181 agccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct
      241 gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg
      301 gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct
      361 ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac
      421 gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt
      481 ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag
      541 actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc
      601 atcatggatc aactgcatga agttccggtt ggcaaatcca tcggacttaa gaaaatccag
      661 gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc
      721 ccatcctgta tatcggcgcc tcttgagctg tttcatgact ttgtcgtcga agagatgagc
      781 gtaag
//
