LOCUS       sequence-116464-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS resistant to siRNA treatment by s21729
            (Applied Biosystems) for stable cell line generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    143..167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttaaact
       61 taagcttggt accatggact acaaggacga cgatgacaag tacccttatg acgtgcccga
      121 ttacgctttg aaccagatat caacaagttt gtacaaaaaa gcaggctcca ccatgggaac
      181 caattcagtc gacatgaaca acggcggcaa agccgagaag gagaacaccc cgagcgaggc
      241 caaccttcag gaggaggagg tccggaccct atttgtcagt ggccttcctc tggacattaa
      301 acctcgggag ctctatctgc ttttcagacc atttaagggc tatgagggtt ctcttataaa
      361 gctcacatct aaacagcctg taggttttgt cagttttgac agtcgctcag aagcagaggc
      421 tgcaaagaat gctttgaatg gcatccgctt cgatcctgaa attccgcaaa cactacgact
      481 agagtttgct aaggcaaaca cgaagatggc caagaacaaa ctcgtaggga ctccaaaccc
      541 cagtactcct ctgcccaaca ctgtacctca gttcattgcc agagagccat atgagctcac
      601 agtgcctgca ctttacccca gtagccctga agtgtgggcc ccgtaccctc tgtacccagc
      661 ggagttagcg cctgctctac ctcctcctgc tttcacctat cccgcttcac tgcatgccca
      721 gatgcgctgg ctccctccct ccgaggctac ttctcagggc tggaagtccc gtcagttctg
      781 ctgagc
//
