LOCUS       sequence-116466-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd 
            generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes 
            NI, Feist AM, Vander Heiden MG, Metallo CM
  TITLE     Tracing compartmentalized NADPH metabolism in the cytosol and 
            mitochondria of mammalian cells.
  JOURNAL   Mol Cell. 2014 Jul 17;55(2):253-63. doi: 
            10.1016/j.molcel.2014.05.008. Epub 2014 May 29.
  PUBMED    24882210
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.05.008"; journalName: "Mol Cell"; date: 
            "2014-07-17- 17"; volume: "55"; issue: "2"; pages: "253-63"
COMMENT     Sequence Label: pSLIK-IDH1-R132H-FLAG
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    144..168
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(189..254)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 acaggacgct cgctgcgccc ttcgtctgac gtggcagcgc tcgccgtgag gaggggggcg
       61 cccgcgggag gcgccaaaac ccggcgcgga ggccagatct tgggtgggtt aattaaccat
      121 ctttatggct cgagatgtta tcaaccactt tgtacaagaa agctgggtct agatatctcg
      181 agtcactact tgtcatcgtc atccttgtag tcgatgtcat gatctttata atcaccgtca
      241 tggtctttgt agtcagcccg ggatcctcta gaataaagtt tggcctgagc tagtttgatc
      301 ttcaagtttt ctccaagttt atccatgaac tcaaatgtat tcaagtagtc agaacgttgc
      361 acattgggta aacctttaat gcaagcagcc aagtccttgg tcatgaagcc agcctcaatt
      421 gtctcaatag agacttcttc caaagcattt gcaaagaagg caagctcttt attgttatca
      481 agctttgctc tgtgggctaa ccctctggtc caggcaaaaa tggaagcaat gggattggtg
      541 gacgtctcct gtcctttctg gtacatgcgg tagtgacggg ttacagtccc gtgggcagcc
      601 tctgcttcta ctgtcttgcc atctggacaa accagcacgc tggtcatcat g
//
