LOCUS       sequence-116469-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  TITLE     Unpublished pBGSA FPR1 fluorophore tagged CDNAs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBZSA FPR1 ECFP
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(2..715,716..718,719..721))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(47..68)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     396..415
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
       61 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      121 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      181 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
      241 cgttcttctg cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct
      301 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      361 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      421 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      481 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc ccaggtcagg gtggtcacga
      541 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      601 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      661 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
      721 tgggatcctt tgcctgtaac tccacctctg cagaaggtaa agtagaattg gt
//
